Liquid-microjet photoelectron spectroscopy of the green fluorescent protein chromophore.

ABSTRACT: Green fluorescent protein (GFP), the most widely used fluorescent protein for in vivo monitoring of biological processes, is known to undergo photooxidation reactions. However, the most fundamental property underpinning photooxidation, the electron detachment energy, has only been measured for the deprotonated GFP chromophore in the gas phase. Here, we use multiphoton ultraviolet photoelectron spectroscopy in a liquid-microjet and high-level quantum chemistry calculations to determine the electron detachment energy of the GFP chromophore in aqueous solution. The aqueous environment is found to raise the detachment energy by around 4 eV compared to the gas phase, similar to calculations of the chromophore in its native protein environment. In most cases, electron detachment is found to occu

SUBMITTER: Tau O 

PROVIDER: S-EPMC8791993 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature