Single-Cell Transcriptomics Reveals a Conserved Metaplasia Program in Pancreatic Injury.

ABSTRACT:

Background & aims

Acinar to ductal metaplasia (ADM) occurs in the pancreas in response to tissue injury and is a potential precursor for adenocarcinoma. The goal of these studies was to define the populations arising from ADM, the associated transcriptional changes, and markers of disease progression.

Methods

Acinar cells were lineage-traced with enhanced yellow fluorescent protein (EYFP) to follow their fate post-injury. Transcripts of more than 13,000 EYFP+ cells were determined using single-cell RNA sequencing (scRNA-seq). Developmental trajectories were generated. Data were compared with gastric metaplasia, Kras^G12D-induced neoplasia, and human pancreatitis. Results were confirmed by immunostaining and electron microscopy. Kras^G12D was expressed in

SUBMITTER: Ma Z 

PROVIDER: S-EPMC8792222 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature