In vivo multimodal imaging of hyaluronan-mediated inflammatory response in articular cartilage.

ABSTRACT:

Objective

One driving factor in the progression to posttraumatic osteoarthritis (PTOA) is the perpetuation of the inflammatory response to injury into chronic inflammation. Molecular imaging offers many opportunities to complement the sensitivity of current imaging modalities with molecular specificity. The goal of this study was to develop and characterize agents to image hyaluronan (HA)-mediated inflammatory signaling.

Design

We developed optical (Cy5.5-P15-1) and magnetic resonance contrast agents (Gd-DOTA-P15-1) based in a hyaluronan-binding peptide (P15-1) that has shown anti-inflammatory effects on human chondrocytes, and validated them in vitro and in vivo in two animal models of PTOA.

Results

In vitro studies with a near infrared (NIR) Cy5.5-P15-1 imaging agen

SUBMITTER: Ruiz A 

PROVIDER: S-EPMC8792232 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature