Genetic deletion of platelet PAR4 results in reduced thrombosis and impaired hemostatic plug stability.

ABSTRACT:

Background

Protease-activated receptor 4 (PAR4) is expressed by a wide variety of cells, including megakaryocytes/platelets, immune cells, cardiomyocytes, and lung epithelial cells. It is the only functional thrombin receptor on murine platelets. A global deficiency of PAR4 is associated with impaired hemostasis and reduced thrombosis.

Objective

We aimed to generate a mouse line with a megakaryocyte/platelet-specific deletion of PAR4 (PAR4^fl/fl ;PF4^Cre+ ) and use the mouse line to investigate the role of platelet PAR4 in hemostasis and thrombosis in mice.

Methods

Platelets from PAR4^fl/fl ;PF4^Cre+ were characterized in vitro. Arterial and venous thrombosis was analyzed. Hemostatic plug formation was analyzed using a saphenous

SUBMITTER: Lee RH 

PROVIDER: S-EPMC8792346 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature