Development of an Indirect ELISA to Detect African Swine Fever Virus pp62 Protein-Specific Antibodies.

ABSTRACT: African swine fever (ASF) is a highly detrimental viral disease caused by African swine fever virus (ASFV). The occurrence and prevalence of this disease have become a serious threat to the global swine industry and national economies. At present, the detection volume of African swine fever is huge, more sensitive and accurate detection techniques are needed for the market. pp62 protein, as a protein in the late stage of infection, has strong antigenicity and a high corresponding antibody titer in infected pigs. In this study, the CP530R gene was cloned into expression vector pET-28a to construct a prokaryotic expression plasmid, which was induced by IPTG to express soluble pp62 protein. Western blot analysis showed that it had great reactivity. Using the purified recombinant protein as an

SUBMITTER: Zhong K 

PROVIDER: S-EPMC8792508 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature