Understanding the Adhesion Mechanism of Hydroxyapatite-Binding Peptide.

ABSTRACT: Understanding the interactions between the protein collagen and hydroxyapatite is of high importance for understanding biomineralization and bone formation. Here, we undertook a reductionist approach and studied the interactions between a short peptide and hydroxyapatite. The peptide was selected from a phage-display library for its high affinity to hydroxyapatite. To study its interactions with hydroxyapatite, we performed an alanine scan to determine the contribution of each residue. The interactions of the different peptide derivatives were studied using a quartz crystal microbalance with dissipation monitoring and with single-molecule force spectroscopy by atomic force microscopy. Our results suggest that the peptide binds via electrostatic interactions between cationic moieties of the

SUBMITTER: Duanis-Assaf T 

PROVIDER: S-EPMC8793143 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature