Progesterone differentially affects the transcriptomic profiles of cow endometrial cell types.

ABSTRACT:

Background

The endometrium is a heterogeneous tissue composed of luminal epithelial (LE), glandular epithelial (GE), and stromal cells (ST), experiencing progesterone regulated dynamic changes during the estrous cycle. In the cow, this regulation at the transcriptomic level was only evaluated in the whole tissue. This study describes specific gene expression in the three types of cells isolated from endometrial biopsies following laser capture microdissection and the transcriptome changes induced by progesterone in GE and ST cells.

Results

Endometrial LE, GE, and ST cells show specific transcriptomic profiles. Most of the differentially expressed genes (DEGs) in response to progesterone are cell type-specific (96%). Genes involved in cell cycle and nuclear division are under

SUBMITTER: Pereira G 

PROVIDER: S-EPMC8793221 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature