Amoxicillin Haptenation of α-Enolase is Modulated by Active Site Occupancy and Acetylation.

ABSTRACT: Allergic reactions to antibiotics are a major concern in the clinic. ß-lactam antibiotics are the class most frequently reported to cause hypersensitivity reactions. One of the mechanisms involved in this outcome is the modification of proteins by covalent binding of the drug (haptenation). Hence, interest in identifying the corresponding serum and cellular protein targets arises. Importantly, haptenation susceptibility and extent can be modulated by the context, including factors affecting protein conformation or the occurrence of other posttranslational modifications. We previously identified the glycolytic enzyme α-enolase as a target for haptenation by amoxicillin, both in cells and in the extracellular milieu. Here, we performed an in vitro study to analyze amoxic

SUBMITTER: Gonzalez-Morena JM 

PROVIDER: S-EPMC8793629 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature