The flagellar motor protein FliL forms a scaffold of circumferentially positioned rings required for stator activation.

ABSTRACT: The flagellar motor stator is an ion channel nanomachine that assembles as a ring of the MotA₅MotB₂ units at the flagellar base. The role of accessory proteins required for stator assembly and activation remains largely enigmatic. Here, we show that one such assembly factor, the conserved protein FliL, forms an integral part of the Helicobacter pylori flagellar motor in a position that colocalizes with the stator. Cryogenic electron tomography reconstructions of the intact motor in whole wild-type cells and cells lacking FliL revealed that the periplasmic domain of FliL (FliL-C) forms 18 circumferentially positioned rings integrated with the 18 MotAB units. FliL-C formed partial rings in the crystal, and the crystal structure-based full ring model was consisten

SUBMITTER: Tachiyama S 

PROVIDER: S-EPMC8794807 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature