Spatiotemporal analysis identifies ABF2 and ABF3 as key hubs of endodermal response to nitrate.

ABSTRACT: Nitrate is a nutrient and a potent signal that impacts global gene expression in plants. However, the regulatory factors controlling temporal and cell type-specific nitrate responses remain largely unknown. We assayed nitrate-responsive transcriptome changes in five major root cell types of the Arabidopsis thaliana root as a function of time. We found that gene-expression response to nitrate is dynamic and highly localized and predicted cell type-specific transcription factor (TF)-target interactions. Among cell types, the endodermis stands out as having the largest and most connected nitrate-regulatory gene network. ABF2 and ABF3 are major hubs for transcriptional responses in the endodermis cell layer. We experimentally validated TF-target interactions for ABF2 and ABF3 by chromat

SUBMITTER: Contreras-Lopez O 

PROVIDER: S-EPMC8794810 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature