Ca²⁺ leak through ryanodine receptor 1 regulates thermogenesis in resting skeletal muscle.

ABSTRACT: Mammals rely on nonshivering thermogenesis (NST) from skeletal muscle so that cold temperatures can be tolerated. NST results from activity of the sarcoplasmic reticulum (SR) Ca²⁺ pump in skeletal muscle, but the mechanisms that regulate this activity are unknown. Here, we develop a single-fiber assay to investigate the role of Ca²⁺ leak through ryanodine receptor 1 (RyR1) to generate heat at the SR Ca²⁺ pump in resting muscle. By inhibiting a subpopulation of RyR1s in a single-fiber preparation via targeted delivery of ryanodine through transverse tubules, we achieve in-preparation isolation of RyR1 Ca²⁺ leak. This maneuver provided a critical increase in signal-to-noise of the SR-temperature-sensitive dye ER thermoyellow fluorescence signal fro

SUBMITTER: Meizoso-Huesca A 

PROVIDER: S-EPMC8794839 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature