Non-telecentric two-photon microscopy for 3D random access mesoscale imaging.

ABSTRACT: Diffraction-limited two-photon microscopy permits minimally invasive optical monitoring of neuronal activity. However, most conventional two-photon microscopes impose significant constraints on the size of the imaging field-of-view and the specific shape of the effective excitation volume, thus limiting the scope of biological questions that can be addressed and the information obtainable. Here, employing a non-telecentric optical design, we present a low-cost, easily implemented and flexible solution to address these limitations, offering a several-fold expanded three-dimensional field of view. Moreover, rapid laser-focus control via an electrically tunable lens allows near-simultaneous imaging of remote regions separated in three dimensions and permits the bending of imaging planes to fo

SUBMITTER: Janiak FK 

PROVIDER: S-EPMC8795402 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature