Dissection of brain-wide resting-state and functional somatosensory circuits by fMRI with optogenetic silencing.

ABSTRACT: To further advance functional MRI (fMRI)-based brain science, it is critical to dissect fMRI activity at the circuit level. To achieve this goal, we combined brain-wide fMRI with neuronal silencing in well-defined regions. Since focal inactivation suppresses excitatory output to downstream pathways, intact input and suppressed output circuits can be separated. Highly specific cerebral blood volume-weighted fMRI was performed with optogenetic stimulation of local GABAergic neurons in mouse somatosensory regions. Brain-wide spontaneous somatosensory networks were found mostly in ipsilateral cortical and subcortical areas, which differed from the bilateral homotopic connections commonly observed in resting-state fMRI data. The evoked fMRI responses to somatosensory stimulation in regions of t

SUBMITTER: Jung WB 

PROVIDER: S-EPMC8795561 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature