A High-Throughput Screening System for Populus Wood-Associated Transcription Factors and Its Application to Lignin Regulation.

ABSTRACT: Wood formation of trees is a complex and costly developmental process, whose regulatory network is involved in the protein-protein and protein-DNA interactions. To detect such interactions in wood development, we developed a high-throughput screening system with 517 Gal4-AD-wood-associated transcription factors (TFs) library from Populus alba × P. glandulosa cv "84K." This system can be used for screening the upstream regulators and interacting proteins of targets by mating-based yeast-one hybrid (Y1H) and yeast-two-hybrid (Y2H) method, respectively. Multiple regulatory modules of lignin biosynthesis were identified based on this Populus system. Five TFs interacted with the 500-bp promoter fragment of PHENYLALANINE AMMONIA-LYASE 2 (PAL2), the first rate-l

SUBMITTER: Zhuang Y 

PROVIDER: S-EPMC8795814 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature