Novel Circulating and Tissue Monocytes as Well as Macrophages in Pancreatitis and Recovery.

ABSTRACT:

Background and aims

Acute pancreatitis (AP) is an inflammatory disease with mild to severe course that is associated with local and systemic complications and significant mortality. Uncovering inflammatory pathways that lead to progression and recovery will inform ways to monitor and/or develop effective therapies.

Methods

We performed single-cell mass Cytometry by Time Of Flight (CyTOF) analysis to identify pancreatic and systemic inflammatory signals during mild AP (referred to as AP), severe AP (SAP), and recovery using 2 independent experimental models and blood from patients with AP and recurrent AP. Flow cytometric validation of monocytes subsets identified using CyTOF analysis was performed independently.

Results

Ly6C⁺ inflammatory monocytes were the

SUBMITTER: Manohar M 

PROVIDER: S-EPMC8796698 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature