Girdin knockdown promotes apoptosis in colorectal cancer via miR-29c-3p/Girdin axis.

ABSTRACT:

Background

In recent years, the incidence and mortality of colorectal cancer (CRC) have increased year by year among young people. Increased levels of Girdin expression predict a poor prognosis of CRC, which presents a serious threat to human health globally. Herein, we investigated the role of Girdin in CRC and explored the underlying mechanisms in CRC.

Methods

The expression of Girdin was detected in human specimens. HCT116 cells with stably expressing or knock-out Girdin protein were successfully constructed to observe the biological function of gene. Protein expression was determined by immunohistochemistry, immunofluorescence and western blot.

Results

Clinically, overexpression of Girdin was observed in the tumor tissue and poor prognosis of CRC patients. Gain-of

SUBMITTER: Wang J 

PROVIDER: S-EPMC8797721 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature