Tanshinone IIA induces autophagy in colon cancer cells through MEK/ERK/mTOR pathway.

ABSTRACT:

Background

Colon cancer is a common malignancy of the digestive tract. The search for effective drugs to treat colon cancer has become the focus of current researches. Tanshinone IIA (Tan IIA) is a fat-soluble component extracted from tanshinone, a traditional Chinese medicine. Tan IIA can modulate the occurrence and development of tumors, but its effect on autophagy in colon cancer cells has not been reported.

Methods

Two types of colon cancer cell lines were selected and different concentrations of Tan IIA were used to treat cells at different time points. Cell Counting Kit-8 assay (CCK-8) was used to detect the effect of Tan IIA on cell proliferation; transmission electron microscopy was used to observe the formation of autophagosomes; reverse transcription-polymerase cha

SUBMITTER: Qian J 

PROVIDER: S-EPMC8797932 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature