Super-enhancers modulate interleukin-6 expression and function in cancers.

ABSTRACT:

Background

It is widely accepted that inflammatory cytokine, interleukin 6 (IL-6), was not only elevated in cancer but also important in carcinogenesis. But how did IL-6 be produced in tumor microenvironment remains to be addressed.

Methods

Both bioinformatics tools and quantitative real time polymerase chain reaction (RT-PCR) were used to examine the expression of IL-6 in cancer cells. To map super-enhancers of IL-6, sgRNAs were constructed. Stable knockout cells were established and subsequently used for cell proliferation and colony formation assay. The correlation between mapped super-enhancers and IL-6 expression was studied by ATAC-seq analysis.

Results

The expression of IL-6 was high in multiple cancers, including pancreatic cancer (PAAD). The elevated expressi

SUBMITTER: Bao Y 

PROVIDER: S-EPMC8798342 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature