LncRNA FAM66C inhibits pancreatic cancer progression by sponging miR-574-3p.

ABSTRACT:

Background

Pancreatic cancer is an extensively concerned human malignancy around the globe, yet the potential therapeutic target remains to be further determined. MicroRNA and LncRNA have been reported to be involved in progression of pancreatic cancer, while the biological role of microRNA-574-3p (miR-574-3p) and FAM66C in pancreatic cancer development is poorly investigated.

Methods

Quantitative real-time PCR (qPCR) analysis was employed to detect the expression of miR-574-3p and FAM66C in pancreatic normal or cancerous tissues and cells. The proliferative and apoptosis signaling molecules were also examined via qPCR and western blot separately. Additionally, cell proliferation and apoptosis assay were performed via CCK8, colony formation and Annexin V-FITC apoptosis assay

SUBMITTER: Zhu J 

PROVIDER: S-EPMC8798657 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature