Combination of Immunofluorescence and Quantitative Fluorescence <i>In-situ</i> Hybridization for Analysing Differential Gene Expression in the Niche Cells of the <i>Drosophila</i> Lymph Gland.

ABSTRACT: The Drosophila larval haematopoietic organ or lymph gland consists of multiple cell types arranged in zones. The smallest stem cell compartment consists of 40-45 cells that constitute the haematopoietic niche. In order to analyse the haematopoietic niche, it needs to be labelled with a specific antibody to differentiate it from the other cell types. To characterise a phenotype, it is often necessary to investigate the expression of a gene in a particular stem cell compartment within the lymph gland. In such a situation, in-situ hybridization is performed, as it indicates the localization of gene expression. Although chromogenic in-situ hybridization enables us to compare the signal and tissue morphology simultaneously, it fails to harness the information related to the

SUBMITTER: Ramesh P 

PROVIDER: S-EPMC8799906 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature