Transcriptome profiling identifies immune response genes against porcine reproductive and respiratory syndrome virus and Haemophilus parasuis co-infection in the lungs of piglets.

ABSTRACT:

Background

Co-infections of the porcine reproductive and respiratory syndrome virus (PRRSV) and the Haemophilus parasuis (HPS) are severe in Chinese pigs, but the immune response genes against co-infected with 2 pathogens in the lungs have not been reported.

Objectives

To understand the effect of PRRSV and/or HPS infection on the genes expression associated with lung immune function.

Methods

The expression of the immune-related genes was analyzed using RNA-sequencing and bioinformatics. Differentially expressed genes (DEGs) were detected and identified by quantitative real-time polymerase chain reaction (qRT-PCR), immunohistochemistry (IHC) and western blotting assays.

Results

All experimental pigs showed clinical symptoms and lung lesions. RNA-seq analy

SUBMITTER: Zhang J 

PROVIDER: S-EPMC8799943 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature