Xylazole inhibits NO-cGMP pathway in fetal rat nerve cells.

ABSTRACT:

Background

Xylazole (Xyl) is a veterinary anesthetic that is structurally and functionally similar to xylazine. However, the effects of Xyl in vitro remain unknown.

Objectives

This study aimed to investigate the anesthetic mechanism of Xyl using fetal rat nerve cells treated with Xyl.

Methods

Fetal rat nerve cells cultured for seven days were treated with 10, 20, 30, and 40 μg/ mL Xyl for 0, 5, 10, 15, 20, 25, 30, 45, 60, 90, and 120 min. Variations of amino acid neurotransmitters (AANTs), Nitric oxide-Cyclic GMP (NO-cGMP) signaling pathway, and ATPase were evaluated.

Results

Xyl decreased the levels of cGMP and NO in nerve cells. Furthermore, Xyl affected the AANT content and Na⁺-K⁺-ATPase and Ca²⁺-Mg²⁺-ATPase activi

SUBMITTER: Wang X 

PROVIDER: S-EPMC8799944 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature