Demethylation at enhancer upregulates MCM2 and NUP37 expression predicting poor survival in hepatocellular carcinoma patients.

ABSTRACT:

Background

Identification of novel biomarker is important for development of molecular-targeted therapy agents for patients with hepatocellular carcinoma (HCC). This study aims to identify potential prognostic biomarkers and investigate epigenetic mechanism of HCC development.

Methods

Public bulk-RNA seq datasets and proteomic dataset were screened for identification of potential prognostic biomarkers for HCC patients. Public methylomic datasets were analyzed for deciphering the epigenetic mechanism regulating HCC-associated gene expression. Immunoblotting, immunohistochemistry, real-time PCR, and pyrosequencing were used to validate the findings from bioinformatic analyses.

Results

Minichromosome maintenance complex component 2 (MCM2) and nucleoporin 37 (NUP37) were

SUBMITTER: Tang Z 

PROVIDER: S-EPMC8800332 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature