Detection of Two Copies of a bla _NDM-1-Encoding Plasmid in Escherichia coli Isolates from a Pediatric Patient with Diarrhea.

ABSTRACT:

Purpose

To elucidate the contribution of a transferable plasmid harboring the bla _NDM-1 gene in an Escherichia coli clinical isolate to the spread of resistance determinants.

Methods

Nine extended-spectrum β-lactamase-producing E. coli were collected from diarrhea samples from a pediatric patient and genetic linkage was investigated through enterobacteriaceae repetitive intragenic consensus polymerase chain reaction (PCR). Bacterial species were identified by 16s rRNA sequencing, susceptibility testing with the use of a BD Phoenix^TM-100 Automated Microbiology System, and assessment of virulence genes by PCR. The transferability of bla _NDM-1 in E. coli strain TCM3e1 was confirmed by conjugation experiments. C

SUBMITTER: Jiang BW 

PROVIDER: S-EPMC8801394 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature