Enhanced production of poly-γ-glutamic acid via optimizing the expression cassette of Vitreoscilla hemoglobin in Bacillus licheniformis.

ABSTRACT: Poly-γ-glutamic acid (γ-PGA) is a natural polymer with various applications, and its high-viscosity hinders oxygen transmission and improvement of synthesis level. Vitreoscilla hemoglobin (VHB) has been introduced into various hosts as oxygen carrier, however, its expression strength and contact efficiency with oxygen hindered efficient oxygen transfer and metabolite synthesis. Here, we want to optimize the expression cassette of VHB for γ-PGA production. Firstly, our results implied that γ-PGA yields were enhanced when introducing twin-arginine translocation (Tat) signal peptides (SP_YwbN, SP_PhoD and SP_TorA) into VHB expression cassette, and the best performance was attained by SP_YwbN from Bacillus subtilis, the γ-PGA yield of which

SUBMITTER: Zhang Q 

PROVIDER: S-EPMC8801620 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature