Prophage Gene Rv2650c Enhances Intracellular Survival of Mycobacterium smegmatis.

ABSTRACT:

Background

Induced by the pathogen Mycobacterium tuberculosis, tuberculosis remains one of the most dangerous infectious diseases in the world. As a special virus, prophage is domesticated by its host and are major contributors to virulence factors for bacterial pathogenicity. The function of prophages and their genes in M. tuberculosis is still unknown.

Methods

Rv2650c is a prophage gene in M. tuberculosis genome. We constructed recombinant Mycobacterium smegmatis (M. smegmatis) to observe bacteria morphology and analyze the resistance to various adverse environments. Recombinant and control strains were used to infect macrophages, respectively. Furthermore, we performed ELISA experiments of infected macrophages.

Results

Rv26

SUBMITTER: Fan X 

PROVIDER: S-EPMC8801708 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature