Elucidating Spatially-Resolved Changes in Host Signaling During Plasmodium Liver-Stage Infection.

ABSTRACT: Upon transmission to the human host, Plasmodium sporozoites exit the skin, are taken up by the blood stream, and then travel to the liver where they infect and significantly modify a single hepatocyte. Low infection rates within the liver have made proteomic studies of infected hepatocytes challenging, particularly in vivo, and existing studies have been largely unable to consider how protein and phosphoprotein differences are altered at different spatial locations within the heterogeneous liver. Using digital spatial profiling, we characterized changes in host signaling during Plasmodium yoelii infection in vivo without disrupting the liver tissue. Moreover, we measured alterations in protein expression around infected hepatocytes and identified a subset of CD1

SUBMITTER: Glennon EKK 

PROVIDER: S-EPMC8801743 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature