Systematic Determination of TCR-Antigen and Peptide-MHC Binding Kinetics among Field Variants of a Theileria parva Polymorphic CTL Epitope.

ABSTRACT: CTLs are known to contribute to immunity toward Theileria parva, the causative agent of East Coast fever. The Tp9_67-75 CTL epitope from the Muguga strain of T. parva is polymorphic in other parasite strains. Identifying the amino acids important for MHC class I binding, as well as TCR recognition of epitopes, can allow the strategic selection of Ags to induce cellular immunity toward T. parva In this study, we characterized the amino acids important for MHC class I binding and TCR recognition in the Tp9_67-75 epitope using alanine scanning and a series of variant peptide sequences to probe these interactions. In a peptide-MHC class I binding assay, we found that the amino acids at positions 1, 2, and 3 were critical for binding to its restricting M

SUBMITTER: Svitek N 

PROVIDER: S-EPMC8802549 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature