Dataset Information

Revisiting the Role of ß-Tubulin in Drosophila Development: β-tubulin60D is not an Essential Gene, and its Novel Pin ¹ Allele has a Tissue-Specific Dominant-Negative Impact.

ABSTRACT: Diversity in cytoskeleton organization and function may be achieved through alternative tubulin isotypes and by a variety of post-translational modifications. The Drosophila genome contains five different β-tubulin paralogs, which may play an isotype tissue-specific function in vivo. One of these genes, the β-tubulin60D gene, which is expressed in a tissue-specific manner, was found to be essential for fly viability and fertility. To further understand the role of the β-tubulin60D gene, we generated new β-tubulin60D null alleles (β-tubulin60D ^M ) using the CRISPR/Cas9 system and found that the homozygous flies were viable and fertile. Moreover, using a combination of genetic complementation tests, rescue experiments,

SUBMITTER: Krishnan RK

PROVIDER: S-EPMC8802551 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

ACCESS DATA

Dataset Information

Revisiting the Role of ß-Tubulin in Drosophila Development: β-tubulin60D is not an Essential Gene, and its Novel Pin ¹ Allele has a Tissue-Specific Dominant-Negative Impact.

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

Revisiting the Role of ß-Tubulin in Drosophila Development: β-tubulin60D is not an Essential Gene, and its Novel Pin 1 Allele has a Tissue-Specific Dominant-Negative Impact.

OmicsDI is part of the ELIXIR infrastructure

Tweets

Revisiting the Role of ß-Tubulin in Drosophila Development: β-tubulin60D is not an Essential Gene, and its Novel Pin ¹ Allele has a Tissue-Specific Dominant-Negative Impact.