Characterisation of cold-induced mitochondrial fission in porcine aortic endothelial cells.

ABSTRACT:

Background

Previously, we observed that hypothermia, widely used for organ preservation, elicits mitochondrial fission in different cell types. However, temperature dependence, mechanisms and consequences of this cold-induced mitochondrial fission are unknown. Therefore, we here study cold-induced mitochondrial fission in endothelial cells, a cell type generally displaying a high sensitivity to cold-induced injury.

Methods

Porcine aortic endothelial cells were incubated at 4-25 °C in modified Krebs-Henseleit buffer (plus glucose to provide substrate and deferoxamine to prevent iron-dependent hypothermic injury).

Results

Cold-induced mitochondrial fission occurred as early as after 3 h at 4 °C and at temperatures below 21 °C, and was more marked after longer cold incub

SUBMITTER: Quiring L 

PROVIDER: S-EPMC8802553 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature