Cardiac SARS-CoV-2 infection is associated with pro-inflammatory transcriptomic alterations within the heart.

ABSTRACT:

Aims

Cardiac involvement in COVID-19 is associated with adverse outcome. However, it is unclear whether cell-specific consequences are associated with cardiac SARS-CoV-2 infection. Therefore, we investigated heart tissue utilizing in situ hybridization, immunohistochemistry, and RNA-sequencing in consecutive autopsy cases to quantify virus load and characterize cardiac involvement in COVID-19.

Methods and results

In this study, 95 SARS-CoV-2-positive autopsy cases were included. A relevant SARS-CoV-2 virus load in the cardiac tissue was detected in 41/95 deceased (43%). Massive analysis of cDNA ends (MACE)-RNA-sequencing was performed to identify molecular pathomechanisms caused by the infection of the heart. A signature matrix was generated based on the single-cell dataset

SUBMITTER: Brauninger H 

PROVIDER: S-EPMC8803085 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature