Inhibition of ABCC1 Decreases cAMP Egress and Promotes Human Airway Smooth Muscle Cell Relaxation.

ABSTRACT: In most living cells, the second-messenger roles for adenosine 3',5'-cyclic monophosphate (cAMP) are short-lived, confined to the intracellular space, and tightly controlled by the binary switch-like actions of Gα_s (stimulatory G protein)-activated adenylyl cyclase (cAMP production) and cAMP-specific PDE (cAMP breakdown). Here, by using human airway smooth muscle (HASM) cells in culture as a model, we report that activation of the cell-surface β₂AR (β₂-adrenoceptor), a G_s-coupled GPCR (G protein-coupled receptor), evokes cAMP egress to the extracellular space. Increased extracellular cAMP levels ([cAMP]_e) are long-lived in culture and are induced by receptor-dependent and receptor-independent mechanisms in such a way as to define a uni

SUBMITTER: Cao G 

PROVIDER: S-EPMC8803359 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature