Gene activation via Cre/lox-mediated excision in cowpea (Vigna unguiculata).

ABSTRACT:

Key message

Expression of Cre recombinase by AtRps5a_pro or AtDD45_pro enabled Cre/lox-mediated recombination at an early embryonic developmental stage upon crossing, activating transgenes in the hybrid cowpea and tobacco. Genetic engineering ideally results in precise spatiotemporal control of transgene expression. To activate transgenes exclusively in a hybrid upon fertilization, we evaluated a Cre/lox-mediated gene activation system with the Cre recombinase expressed by either AtRps5a or AtDD45 promoters that showed activity in egg cells and young embryos. In crosses between Cre recombinase lines and transgenic lines harboring a lox-excision reporter cassette with ZsGreen driven by the AtUbq3 promoter after Cre/lox-mediated recombination, we observed complete

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC8803690 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature