Impact of the mouse model and molar amount of injected ligand on the tissue distribution profile of PSMA radioligands.

ABSTRACT:

Purpose

Various preclinical study designs are described in the literature for the evaluation of PSMA radioligands. In this study, [¹⁷⁷Lu]Lu-Ibu-DAB-PSMA, an albumin-binding radioligand, and [¹⁷⁷Lu]Lu-PSMA-617 were investigated and compared under variable experimental conditions.

Methods

In vitro cell uptake studies were performed with PC-3 PIP and LNCaP tumor cells using a range of molar concentrations (0.75-500 nM) of both radioligands. Biodistribution and SPECT/CT imaging studies were carried out with the respective tumor mouse models using 0.05 nmol and 1.0 nmol injected ligand per mouse.

Results

In both tumor cell lines, the uptake of the radioligands was increased when using low molar concentrations of the respective ligand. The observed sat

SUBMITTER: Tschan VJ 

PROVIDER: S-EPMC8803738 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature