Differential release of extracellular vesicle tRNA from oxidative stressed renal cells and ischemic kidneys.

ABSTRACT: While urine-based liquid biopsy has expanded to the analyses of extracellular nucleic acids, the potential of transfer RNA (tRNA) encapsulated within extracellular vesicles has not been explored as a new class of urine biomarkers for kidney injury. Using rat kidney and mouse tubular cell injury models, we tested if extracellular vesicle-loaded tRNA and their m¹A (N¹-methyladenosine) modification reflect oxidative stress of kidney injury and determined the mechanism of tRNA packaging into extracellular vesicles. We determined a set of extracellular vesicle-loaded, isoaccepting tRNAs differentially released after ischemia-reperfusion injury and oxidative stress. Next, we found that m¹A modification of extracellular vesicle tRNAs, despite an increase of the me

SUBMITTER: Lee HK 

PROVIDER: S-EPMC8803936 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature