Effective use of sequence information to predict CRISPR-Cas9 off-target

ABSTRACT: Graphical abstract The CRISPR/Cas9 gene-editing system is the third-generation gene-editing technology that has been widely used in biomedical applications. However, off-target effects occurring CRISPR/Cas9 system has been a challenging problem it faces in practical applications. Although many predictive models have been developed to predict off-target activities, current models do not effectively use sequence pair information. There is still room for improved accuracy. This study aims to effectively use sequence pair information to improve the model's performance for predicting off-target activities. We propose a new coding scheme for coding sequence pairs and design a new model called CRISPR-IP for predicting off-target activity. Our coding scheme distinguishes regions with different fu

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PROVIDER: S-EPMC8804193 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature