Highly Efficient One-Step Protein Immobilization on Polymer Membranes Supported by Response Surface Methodology.

ABSTRACT: Immobilization of proteins by covalent coupling to polymeric materials offers numerous excellent advantages for various applications, however, it is usually limited by coupling strategies, which are often too expensive or complex. In this study, an electron-beam-based process for covalent coupling of the model protein bovine serum albumin (BSA) onto polyvinylidene fluoride (PVDF) flat sheet membranes was investigated. Immobilization can be performed in a clean, fast, and continuous mode of operation without any additional chemicals involved. Using the Design of Experiments (DoE) approach, nine process factors were investigated for their influence on graft yield and homogeneity. The parameters could be reduced to only four highly significant factors: BSA concentration, impregnation method,

SUBMITTER: Schmidt M 

PROVIDER: S-EPMC8804297 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature