RNA sequencing of glioblastoma tissue slice cultures reveals the effects of treatment at the transcriptional level.

ABSTRACT: One of the major challenges in cancer research is finding models that closely resemble tumors within patients. Human tissue slice cultures are a promising approach to provide a model of the patient's tumor biology ex vivo. Recently, it was shown that these slices can be successfully analyzed by whole transcriptome sequencing as well as automated histochemistry, increasing their usability as preclinical model. Glioblastoma multiforme (GBM) is a highly malignant brain tumor with poor prognosis and little is known about its genetic background and heterogeneity regarding therapy success. In this study, tissue from the tumors of 25 patients with primary GBM was processed into slice cultures and treated with standard therapy (irradiation and temozolomide). Total RNA sequencing and automated hist

SUBMITTER: Haehnel S 

PROVIDER: S-EPMC8804611 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature