The Chondrogenic Potential of First-Trimester and Term Placental Mesenchymal Stem/Stromal Cells.

ABSTRACT:

Objectives

Mesenchymal stem/stromal cells (MSCs) are a well-established cell source for cartilage engineering, but challenges remain as differentiation often results in chondrocyte hypertrophy. Chondrogenic potential also varies with MSC source and donor age. We assessed the chondrogenic potential of first-trimester and term placental MSCs and compared their response to commonly used bone marrow MSCs (BM-MSCs).

Design

MSCs were isolated from first-trimester and term placentae. BM-MSCs were commercially obtained. Chondrogenesis was induced by micromass culture in commercial chondrogenic media for 7, 14, or 21 days. Pellets were assessed for glycosaminoglycan (GAG) content, and types I, II, and X collagen. Gene expression was profiled using Qiagen RT2 human MSC arrays.

Resu

SUBMITTER: James JL 

PROVIDER: S-EPMC8804733 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature