Macromolecular Interactions in Cartilage Extracellular Matrix Vary According to the Cartilage Type and Location.

ABSTRACT:

Objective

To investigate GAG-ECM (glycosaminoglycan-extracellular matrix) interactions in different cartilage types. To achieve this, we first aimed to determine protocols for consistent calculation of GAG content between cartilage types.

Design

Auricular cartilage containing both collagen and elastin was used to determine the effect of lyophilization on GAG depletion activity. Bovine articular, auricular, meniscal, and nasal cartilage plugs were treated using different reagents to selectively remove GAGs. Sulfated glycosaminoglycan (sGAG) remaining in the sample after treatment were measured, and sGAG loss was compared between cartilage types.

Results

The results indicate that dry weight of cartilage should be measured prior to cartilage treatment in order to provide

SUBMITTER: Rathnayake MSB 

PROVIDER: S-EPMC8804747 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature