Hypertonic Dextrose Stimulates Chondrogenic Cells to Deposit Collagen and Proliferate.

ABSTRACT:

Objective

Hypertonic dextrose (HD) injections (prolotherapy) for osteoarthritis are reported to reduce pain. Cartilage regeneration is hypothesized as a mechanism. This in vitro study identifies an HD concentration that stimulates chondrogenic cells to increase metabolic activity and assesses whether this concentration affects collagen deposition and proliferation.

Design

ATDC5 chondrogenic cells were cultured in normoglycemic DMEM/F12 medium, treated with concentrations of HD (4-400 mM), and assessed with PrestoBlue. Advanced light microscopy was used to conduct live imaging of collagen deposition through second harmonic generation microscopy (SHG) and proliferation via 2-photon excitation microscopy. Proliferation was additionally assessed with hemocytometer counts.

SUBMITTER: Johnston E 

PROVIDER: S-EPMC8804764 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature