Integrative Analysis of MicroRNA and mRNA Sequencing Data Identifies Novel Candidate Genes and Pathways for Developmental Dysplasia of Hip.

ABSTRACT:

Objective

Our aim is to explore the candidate pathogenesis genes and pathways of developmental dysplasia of hip (DDH).

Design

Proliferating primary chondrocytes from hip cartilage were used for total RNA extraction including 5 DDH patients and 5 neck of femur fracture (NOF) subjects. Genome-wide mRNA and microRNA (miRNA) were then sequenced on the Illumina platform (HiSeq2500). Limma package was used for difference analysis of mRNA expression profiles. edgeR was used for difference analysis of miRNA expression profiles. miRanda was used to predict miRNA-target genes. The overlapped DDH associated genes identified by mRNA and miRNA integrative analysis were further compared with the differently expressed genes in hip osteoarthritis (OA) cartilage.

Results

Differential

SUBMITTER: Cheng B 

PROVIDER: S-EPMC8804775 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature