Caspase Inhibition Affects the Expression of Autophagy-Related Molecules in Chondrocytes.

ABSTRACT: Objective. Caspases, cysteine proteases traditionally associated with apoptosis and inflammation, have recently been identified as important regulators of autophagy and reported within the growth plate, a cartilaginous part of the developing bone. The aim of this research was to identify novel autophagy-related molecules affected by inhibition of pro-apoptotic caspases in chondrocytes. Design. Chondrocyte micromasses derived from mouse limb buds were treated with pharmacological inhibitors of caspases. Autophagy-related gene expression was examined and possible novel molecules were confirmed by real-time polymerase chain reaction and immunocytofluorescence. Individual caspases inhibitors were used to identify the effect of specific caspases. Results. Chondrogenesis acc

SUBMITTER: Vesela B 

PROVIDER: S-EPMC8804809 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature