The PI3K/Akt/mTOR pathway as a preventive target in melanoma brain metastasis.

ABSTRACT:

Background

Brain metastases (BM) are a frequent complication of malignant melanoma (MM), with limited treatment options and poor survival. Prevention of BM could be more effective and better tolerated than treating established BM in various conditions.

Methods

To investigate the temporospatial dynamics of PI3K/Akt/mTOR (PAM) pathway activation during BM formation and the preventive potential of its inhibition, in vivo molecular imaging with an Akt biosensor was performed, and long-term intravital multiphoton microscopy through a chronic cranial window in mice.

Results

In vivo molecular imaging revealed invariable PAM pathway activation during the earliest steps of brain colonization. In order to perform a long-term intravascular arrest and to extravasate, circulating

SUBMITTER: Tehranian C 

PROVIDER: S-EPMC8804893 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature