Co-flow injection for serial crystallography at X-ray free-electron lasers.

ABSTRACT: Serial femtosecond crystallography (SFX) is a powerful technique that exploits X-ray free-electron lasers to determine the structure of macro-molecules at room temperature. Despite the impressive exposition of structural details with this novel crystallographic approach, the methods currently available to introduce crystals into the path of the X-ray beam sometimes exhibit serious drawbacks. Samples requiring liquid injection of crystal slurries consume large quantities of crystals (at times up to a gram of protein per data set), may not be compatible with vacuum configurations on beamlines or provide a high background due to additional sheathing liquids present during the injection. Proposed and characterized here is the use of an immiscible inert oil phase to supplement the flow of sampl

SUBMITTER: Doppler D 

PROVIDER: S-EPMC8805165 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature