Lunatic Fringe-GFP Marks Lamina-Specific Astrocytes That Regulate Sensory Processing.

ABSTRACT: Astrocytes are the most abundant glial cell in the brain and perform a wide range of tasks that support neuronal function and circuit activities. There is emerging evidence that astrocytes exhibit molecular and cellular heterogeneity; however, whether distinct subpopulations perform these diverse roles remains poorly defined. Here we show that the Lunatic Fringe-GFP (Lfng-GFP) bacteria artificial chromosome mouse line from both sexes specifically labels astrocyte populations within lamina III and IV of the dorsal spinal cord. Transcriptional profiling of Lfng-GFP⁺ astrocytes revealed unique molecular profiles, featuring an enriched expression of Notch- and Wnt- pathway components. Leveraging CRE-DOG viral tools, we ablated Lfng-GFP⁺ astrocytes, which decreased neurona

SUBMITTER: Akdemir ES 

PROVIDER: S-EPMC8805626 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature