L-carnitine in a certain concentration increases expression of cell surface marker CD34 and apoptosis in the rat bone marrow CD34⁺ hematopoietic stem cells.

ABSTRACT:

Background

Stem cell based therapy has been encouraged as an attractive method in regenerative medicine. Poor survival and maintenance of the cells transferred into the damaged tissue are broadly accepted as serious barriers to enhancing the efficacy of regenerative medicine. For this reason, some antioxidants such as L-carnitine (LC) are used as a favorite strategy to improve cell survival and retention properties.

Aims

This study aims to evaluate the effect of LC on the expression of CD34 marker and its effect on apoptosis and SUZ12 gene expression.

Methods

Rat bone marrow mono-nuclear cells (rBMNCs) were isolated. Then, CD34⁺ hematopoietic stem cells (HSCs) were enriched using the magnetic activated cell sorting (MACS) method. The cells were treat

SUBMITTER: Fathi E 

PROVIDER: S-EPMC8806168 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature