Dataset Information

Phylogenetic analysis of canine parvovirus 2 subtypes from diarrheic dogs in Iran.

ABSTRACT:

Background

Canine parvovirus type 2 (CPV-2) causes gastroenteritis and leukopenia in dogs worldwide. They are three subtypes of CPV-2 including CPV-2a, CPV-2b, and CPV-2c. The distribution status of CPV-2 subtypes has been shown differences in many countries.

Aims

The aim of the present study was detection and phylogenetic analysis of different subtypes of CPV-2 circulating in two provinces of Iran, Tehran and Alborz.

Methods

CPV-2 was detected using 555 primer pairs in collected samples. Phylogenetic analysis of CPV-2 subtypes was done using sequencing of the partial length of VP2 gene.

Results

Twenty-eight CPV-2 were detected using 555 primer pair. The sequences of isolates were deposited in the GenBank database. Phylogenetic analysis revealed that all CPV-2c subtype isolates had very high sequence identity to China and Zambia that form a distinct cluster.

Conclusion

In conclusion, this study revealed the emergence of all CPV-2 variants in dogs in Iran. Thus, the continual monitoring of CPV-2 in domestic dogs should be further conducted on a large scale to determine the predominant variants and their distributions in the country and to follow the dynamics of CPV-2 in the Middle East region of Asia.

SUBMITTER: Ghajari M

PROVIDER: S-EPMC8806170 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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Publications

Phylogenetic analysis of canine parvovirus 2 subtypes from diarrheic dogs in Iran.

Ghajari M M Pourtaghi H H Lotfi M M

Iranian journal of veterinary research 20210101 4

<h4>Background</h4>Canine parvovirus type 2 (CPV-2) causes gastroenteritis and leukopenia in dogs worldwide. They are three subtypes of CPV-2 including CPV-2a, CPV-2b, and CPV-2c. The distribution status of CPV-2 subtypes has been shown differences in many countries.<h4>Aims</h4>The aim of the present study was detection and phylogenetic analysis of different subtypes of CPV-2 circulating in two provinces of Iran, Tehran and Alborz.<h4>Methods</h4>CPV-2 was detected using 555 primer pairs in col ...[more]

PMID: 35126544

Similar Datasets

Project description:AimThis study aimed to detect and characterize current genotypes of canine parvovirus (CPV) in Egypt during 2018.Materials and methodsA total of 50 fecal swabs were collected from clinically infected domestic dogs of 2-5 months of age, suspected to suffer from CPV infection, from Cairo and Giza Governorates. The samples were subjected to qualitative antigen detection using the rapid test, followed by isolation on Madin-Darby Canine Kidney (MDCK) cells, molecular characterization with partial amplification of VP2 gene using polymerase chain reaction (PCR), followed by sequencing and phylogenetic analysis.ResultsOut of 50 fecal samples, 20 samples were positive (40%) by Rapid CPV/canine coronavirus Ag Test Kit. These positive samples were cultured successfully on MDCK cells. Nine randomly chosen samples out of 30 apparently negative samples were amplified using PCR with primers Hfor and Hrev to yield a typical 630 bp fragment. Then, six randomly chosen samples out of nine were amplified using PCR with primers Pbs and Pbas to yield a typical 427 bp fragment. Sequencing, BLAST analysis and assembly of the two fragments (630 bp and 427 bp) to produce 912 bp fragments, in the six samples, revealed two serotypes CPV-2b and CPV-2c. The obtained strains were submitted to GenBank and given accession numbers MK642272, MK642273, MK642274, MK642275, MK642276, and MK642277. Phylogenetic analysis of the Egyptian strains serotype 2b illustrated that they were closely related to Thailand strains (accession numbers KP715709, KP715694, KP715701, and KP715700); while Egyptian strains serotype 2c was closely related to Thailand strains (accession numbers MH711894 and MH711902), Taiwanese strain (KU244254), Chinese strain (MF467242), and Vietnamese strain (accession number LC216910).ConclusionThe current research recommends further epidemiological studies to assess the extent of the occurrence of different serotypes of CPV in Egypt and the efficiency of imported and locally produced vaccines in protection against CPV infection.

Dataset Information

Phylogenetic analysis of canine parvovirus 2 subtypes from diarrheic dogs in Iran.

Background

Aims

Methods

Results

Conclusion

Publications

Phylogenetic analysis of canine parvovirus 2 subtypes from diarrheic dogs in Iran.

Similar Datasets

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