Effect of fed-batch and chemostat cultivation processes of C. glutamicum CP for L-leucine production.

ABSTRACT: Most of the current industrial processes for L-leucine production are based on fermentation, usually in fed-batch operation mode. Although the culture technology has advanced in recent decades, the process still has significant drawbacks. To solve these problems, we investigated the effects of chemostat culture conditions on the production of L-leucine by Corynebacterium glutamicum CP. The dilution rate, the nitrogen source, and the carbon-nitrogen ratio of the medium were optimized. With the addition of ammonium acetate to the chemostat medium, the initial C/N ratio was adjusted to 57.6, and the L-leucine titer reached the highest level at the optimal dilution rate of 0.04 h^-1. Compared with fed-batch culture, the L-leucine titer was reduced (from 53.0 to 24.8 g L^-1

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC8806242 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature