Establishing functional lentiviral vector production in a stirred bioreactor for CAR-T cell therapy.

ABSTRACT: As gene delivery tools, lentiviral vectors (LV) have broad applications in chimeric antigen receptor therapy (CAR-T). Large-scale production of functional LV is limited by the adherent, serum-dependent nature of HEK293T cells used in the manufacturing. HEK293T adherent cells were adapted to suspension cells in a serum-free medium to establish large-scale processes for functional LV production in a stirred bioreactor without micro-carriers. The results showed that 293 T suspension was successfully cultivated in F media (293 CD05 medium and SMM293-TII with 1:1 volume ratio), and the cells retained the capacity for LV production. After cultivation in a 5.5 L bioreactor for 4 days, the cells produced 1.5 ± 0.3 × 10⁷ TU/mL raw LV, and the lentiviral transduction efficiency was 48.6 ±

SUBMITTER: Tang QL 

PROVIDER: S-EPMC8806440 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature